Enzymic determination of plasma cholesterol on discrete automatic analysers

Enzymic procedures for the determination of plasma cholesterol, using cholesterol esterase and cholesterol oxidase, have been adapted to the Vickers D-300, Vickers M,-300, and Vitatron AKES discrete analysers. The results obtained by these methods have been compared to those obtained by manual and continuous flow Liebermann-Burchard methods. The enzymic methods were found to be accurate, precise and of adequate sensitivity.