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Updated: Jul 22, 2026

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
Published on: September 21, 2012
Cell-cell interactions influence oligosaccharide modifications on mucins and other large glycoproteins
P L Stranahan1, J Laroe, R McCombs
1University of Colorado Cancer Center, Denver 80262, USA.
Abstract:
Intratumoral phenotypic diversity is well documented with regard to tumor associated carbohydrate antigens (TACA). The factors which control the expression of these cell-surface oligosaccharides on different cells of the same tumor are not understood. We investigated the expression of a panel of mucin associated oligosaccharides in cell lines growing at different surface densities (number of cells per cm2 of growth flask). Results show that the apparent expression of extended Lea-Lex, Lea and Lex, sialyl Lea, Tn and sialyl Tn varies with density of growth by an invasive human squamous cell lung carcinoma cell line (NU6-1), a benign variant (NE-18) and the human lung epithelial cell line BEAS-2B. The results indicate that one of the factors influencing the apparent expression of mucin-associated oligosaccharides is cell-cell interactions.
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