Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of elevated temperature on cytotoxic effector cells

V K Singh1, S Biswas, C M Pandey

  • 1Department of Immunology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow, India.

Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of appropriate statistical tools in biomedical research: Current trend & status.

The Indian journal of medical research·2023
Same author

Experimental Evidence for an Attractive p-ϕ Interaction.

Physical review letters·2021
Same author

Multiharmonic Correlations of Different Flow Amplitudes in Pb-Pb Collisions at sqrt[s_{NN}]=2.76  TeV.

Physical review letters·2021
Same author

Soft-Dielectron Excess in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2021
Same author

Elliptic Flow of Electrons from Beauty-Hadron Decays in Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2021
Same author

New ^{59}Fe Stellar Decay Rate with Implications for the ^{60}Fe Radioactivity in Massive Stars.

Physical review letters·2021

Elevated temperatures impact immune cell functions differently. While some cytotoxic functions are inhibited, others like monocyte and TNF-mediated cytotoxicity show enhancement, suggesting complex immune responses to fever.

Area of Science:

  • Immunology
  • Cellular Biology
  • Physiology

Background:

  • Immune response is known to correlate with temperature.
  • The impact of elevated temperatures on cytotoxic effector functions remains largely unknown.

Purpose of the Study:

  • To investigate the effect of elevated temperatures (38.5°C and 40°C) on various immune cell cytotoxic functions.
  • To compare these effects with standard physiological temperature (37°C).

Main Methods:

  • Assessed cytotoxicity of lymphokine-activated killer (LAK) cells, monocyte-derived cells, cytotoxic T lymphocytes (CTL), and tumor necrosis factor (TNF)-mediated cytotoxicity.
  • Utilized K562 and Raji cells as targets for natural killer (NK)-sensitive and NK-resistant assays, respectively.
  • Investigated lipopolysaccharide (LPS) effects on monocyte cytotoxicity at different temperatures.

Related Experiment Videos

Main Results:

  • LAK cell cytotoxicity was significantly inhibited at 40°C against NK-sensitive targets.
  • Monocyte cytotoxicity showed non-significant enhancement at both 38.5°C and 40°C, with LPS significantly boosting this effect at both temperatures.
  • CTL cytotoxicity was significantly inhibited at 38.5°C, while TNF-mediated cytotoxicity was significantly enhanced at both elevated temperatures.

Conclusions:

  • Elevated temperatures have differential effects on cytotoxic immune functions.
  • While LAK and CTL functions may be impaired during fever, monocyte and TNF-mediated cytotoxicity could be enhanced, indicating a complex immune regulatory response.