Cloning and expression of mouse UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3

¹Department of Dental Research, School of Medicine and Dentistry, University of Rochester, New York 14642, USA.

Biochemical and Biophysical Research Communications

|November 1, 1996

Abstract:

A novel isoform of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, designated ppGaNTase-T3, has been cloned from a mouse testis cDNA library and expressed in COS7 cells. ppGaNTase-T3 displayed 64 and 59% amino acid identity with ppGaNTase-T1 and ppGaNTase-T2, respectively, and 96% amino acid identity with the recently reported human form of ppGaNTase-T3. The ppGaNTase-T3 transcript is abundant in the major salivary glands, gastrointestinal tract and both the male and female reproductive systems. ppGaNTase-T3 and ppGaNTase-T1 display overlapping substrate preferences in vitro, although mapping studies of O-glycosylated peptides suggests that certain hydroxyamino acids are preferentially glycosylated by each isoform. This suggests that more than one isoform of ppGaNTase may be required to complete the O-glycosylation of endogenous substrates.

Related Experiment Videos

Cloning and expression of mouse UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3

Related Concept Videos

Use of affinity-directed liquid chromatography-mass spectrometry to map the epitopes of a factor VIII inhibitor antibody fraction.

Global oral health inequalities: the view from a research funder.

The role of NELL-1, a growth factor associated with craniosynostosis, in promoting bone regeneration.

Substrate-induced conformational changes and dynamics of UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase-2.

A revolution in biomedical assessment: the development of salivary diagnostics.

Glycopeptide N-acetylgalactosaminyltransferase specificities for O-glycosylated sites on MUC5AC mucin motif peptides.

Stem cells from human exfoliated deciduous teeth as an alternative cell source for cartilage regeneration and repair.

PIST couples actin cytoskeleton dynamics through a WH2-like actin-binding domain.

Refinement and initial characterization of a murine mandibular distraction osteogenesis model.

Engineering ADAR1p150- and dCasRx-based systems for programmable RNA base editing in plants.

Imiquimod triggers TLR7-independent apoptosis but TLR7-dependent activation in murine CD4+ T cells.

Advances in adropin-mediated signaling pathways and molecular mechanisms: Receptor controversies, tissue-specific evidence, and emerging physiological roles.

A Monte Carlo-Driven Genetic Algorithm and Digital Twin Approach for Quantitative X-ray Fluorescence Spectroscopy.

Early improvements in function with atogepant as assessed by the Activity Impairment in Migraine-Diary: Post hoc analyses of three randomized, phase 3 clinical trials of atogepant for the preventive treatment of migraine.

Polynomial smoothing and curvature estimation of noisy biological surfaces with application to aortic morphology.

blenderFaceUV: Toward Marker-Based Precision and Flexibility in Facial Tracking With "Invisible" Markers.

Regional mismatch and coordinated recycling of end-of-life crystalline silicon photovoltaic modules in China.

Methods for measuring stratigraphic attitude and true thickness based on high-precision 3D real-scene models: A case study of the early Silurian Chongqing Lagerstätte Section, China.

Related Experiment Videos

Cloning and expression of mouse UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3

Abstract:

Related Concept Videos

Related Articles