Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

p53 mutations in solar keratoses

W S Park1, H K Lee, J Y Lee

  • 1Department of Pathology, Catholic University Medical College, Seoul, Korea.

Human Pathology
|November 1, 1996
PubMed
Summary

Sunlight exposure causes p53 gene mutations, including point mutations and loss of heterozygosity, in solar keratoses, indicating a role in skin cancer development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NuSTAR as an Axion Helioscope.

Physical review letters·2025
Same author

Multi-Gb/s free-space laser communication at 4.6-μm wavelength using a high-speed, room-temperature, resonant-cavity infrared detector (RCID) and a quantum-cascade laser.

Optics express·2024
Same author

Thickness measurement of nm HfO<sub>2</sub> films.

Metrologia·2024
Same author

Detection of dimethyl methylphosphonate (DMMP) with an interband cascade laser sensor.

Optics express·2024
Same author

Seasonal variability of ocean circulation near the Dotson Ice Shelf, Antarctica.

Nature communications·2022
Same author

Multi-species sensing using multi-mode absorption spectroscopy with mid-infrared interband cascade lasers.

Applied physics. B, Lasers and optics·2020

Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • Solar keratoses are pre-malignant skin lesions.
  • Sunlight is a known carcinogen.
  • The p53 gene is a tumor suppressor crucial for preventing cancer.

Purpose of the Study:

  • To investigate the role of p53 gene mutations in solar keratoses.
  • To determine if sunlight exposure leads to p53 gene alterations.
  • To analyze p53 point mutations, loss of heterozygosity (LOH), and protein overexpression.

Main Methods:

  • Polymerase chain reaction (PCR) amplification and sequencing of p53 exons 4-9.
  • Restriction fragment length polymorphism (RFLP) analysis for LOH at codon 72.
  • Immunohistochemistry using monoclonal antibody (PAb 1801) for p53 protein overexpression.

Main Results:

  • p53 point mutations were found in 28% of solar keratoses, predominantly at pyrimidine bases.
  • Loss of heterozygosity (LOH) was detected in 29% of informative samples.
  • p53 protein overexpression (immunopositivity) was observed in 28% of cases, with varying correlation to mutations.

Conclusions:

  • Sunlight exposure can induce p53 gene mutations in solar keratoses.
  • p53 gene alterations are implicated in the pathogenesis of sunlight-induced skin carcinogenesis.
  • Specific lesion types (bowenoid, hypertrophic) showed characteristic p53 alterations.

Related Experiment Videos