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Updated: Jul 26, 2026
12:34
DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
Sequencing 5-methylcytosine residues by the bisulphite method
1Peptide Technology Limited, at the CSIRO Laboratory of Molecular Biology, Sydney, Australia.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1996
Abstract:
Measuring patterns of cytosine methylation in genomic DNA is most efficiently accomplished by use of the bisulphite method. This method depends on the large difference in reactivity of cytosines relative to 5-Methyl cytosines in genomic DNA to bisulphite. The chemistry and history of the method and recent developments which greatly increase its sensitivity, simplicity and reliability are described. An updated protocol to guide users is appended.
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