Binding assay of low density lipoprotein to Asp-hemolysin from Aspergillus fumigatus

Binding activity of Asp-hemolysin to low density lipoprotein (LDL) was measured. LDL binds to Asp-hemolysin with an affinity as high as the LDL receptor. The apparent Kd, determined by Scatchard plot analysis, was 8.9 x 10(-9) M [125I]LDL. Asp-hemolysin is thus identified as a novel LDL-binding protein.