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Increased deoxycytidine kinase activity by etoposide in L1210 murine leukemic cells
K Ooi1, T Ohkubo, M Higashigawa
1Department of Pharmacy, Yokkaichi Social Insurance Hospital, Mie, Japan.
Biological & Pharmaceutical Bulletin
|October 1, 1996
Abstract:
Deoxycytidine kinase (dCK) is a rate-limiting enzyme for the activation of ara-C. It is responsible for the phosphorylation of ara-C which is widely used in the treatment of leukemia. We examined the effect of etoposide on dCK in L1210 cells and found that incubation with 10 microM etoposide for 1 h increased dCK activity to 170% of the control. This effect of etoposide on dCK activity was concentration-dependent up to at least 100 microM of the substance.