Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Incorporating anomalous scattering centres into macromolecules

H S Pappa1, A E Stewart, N Q McDonald

  • 1Structural Biology Laboratory, Imperial Cancer Research Fund, London, UK.

Current Opinion in Structural Biology
|October 1, 1996
PubMed
Summary

Multiwavelength anomalous diffraction (MAD) phase evaluation is now more accessible for macromolecular structural analysis. New methods for introducing anomalous scattering centers overcome previous limitations, enabling broader application of MAD.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intensity Discrimination of Electrocutaneous Haptic Feedback Is Enhanced at Low Frequencies and with Repeated Exposure.

IEEE transactions on haptics·2026
Same author

Kinome profiling reveals pathogenic variant specific protein signalling networks in MEN2 children with Medullary Thyroid Cancer.

NPJ precision oncology·2025
Same author

Architecture and regulation of a GDNF-GFRα1 synaptic adhesion assembly.

Nature communications·2023
Same author

Activation of the receptor tyrosine kinase RET improves long-term hematopoietic stem cell outgrowth and potency.

Blood·2020
Same author

Mechanisms of RET signaling in cancer: current and future implications for targeted therapy.

Cellular signalling·2014
Same author

A new outbreak of rabies in rare Ethiopian wolves (Canis simensis).

Archives of virology·2010

Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • Maldiffraction (MAD) is a powerful technique for macromolecular phase determination.
  • Previous limitations in introducing anomalous scattering centers restricted MAD's widespread application.

Purpose of the Study:

  • To discuss recent advancements enabling broader use of MAD for macromolecular structural analysis.
  • To highlight new tools for overcoming limitations in anomalous scattering center introduction.

Main Methods:

  • Chemical modification of macromolecules.
  • Protein engineering for introducing scattering centers.
  • Biosynthetic labeling strategies.

Main Results:

Related Experiment Videos

  • Development of versatile methods for incorporating anomalous scatterers into macromolecules.
  • Overcoming prior constraints on the selection of suitable scattering centers.
  • Enabling most structural analyses to utilize the MAD approach.

    • Conclusions:

    • Recent innovations have significantly expanded the applicability of MAD phase evaluation.
    • The described methods facilitate broader adoption of MAD in structural biology.
    • Macromolecular structural determination is now more amenable to MAD techniques.