Optimisation of platelet aggregometry utilising micotitreplate technology and integrated software

The possible applications of the measurement of platelet aggregation by conventional photometry are limited by the short lifetime of platelet suspensions. Recently a microplate-based technique was introduced, which promised to revolutionize experimental design by enabling simultaneous assessment of 96 platelet samples. However, adoption of this technique has been slow since the available equipment was not able to satisfy all the conditions required for conventional aggregometry, especially adequate agitation of samples whilst maintaining total control of incubation temperature. This report describes how these problems may be overcome, together with the introduction of on-line analysis of data using commercially available software, obviating the complexities of data management previously encountered.