Related Experiment Video
Updated: Aug 13, 2026

06:34
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Speech recognition and direct data entry in clinical microbiology
1Southampton Public Health Laboratory, Southampton General Hospital, England, UK.
British Journal of Biomedical Science
|September 1, 1996
Summary
This study introduces Vigilante, a speech-based system for direct entry of urine test results. Vigilante streamlines data entry, enhances accuracy, and reduces reporting time, offering a practical alternative to traditional methods.
Area of Science:
- Medical Laboratory Science
- Health Informatics
Background:
- Traditional data entry for laboratory results is time-consuming and prone to errors.
- Existing methods often involve manual coding and keyboard input, delaying report turnaround times.
Purpose of the Study:
- To design and evaluate a speech-based system (Vigilante) for direct data entry of urine microscopy, culture, and sensitivity test results at the bench level.
- To reduce data entry time, improve accuracy, and expedite report issuance.
Main Methods:
- The Vigilante system was developed for natural continuous speech input, incorporating graphical and audio feedback for error correction.
- A three-month comparative study involving 2360 urine specimens evaluated Vigilante against traditional data entry methods with multiple operators.
Main Results:
- Vigilante significantly reduced data entry time compared to traditional methods.
- The system demonstrated improved accuracy in reporting and decreased overall report issuance time.
- Graphical and audio feedback features facilitated efficient error recognition and correction.
Conclusions:
- Direct data entry using speech technology, as exemplified by the Vigilante system, provides a practical, reliable, and innovative solution for laboratory reporting.
- The Vigilante system is now routinely used for urine test results and is adaptable to other laboratory methodologies and data entry tasks.
Related Concept Videos
Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Key Techniques in Microbiology
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

