Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Measuring infiltration during paraffin wax processing for histology

R T Allison1, D Lloyd

  • 1Department of Oral Surgery, Medicine & Pathology, University of Wales College of Medicine, Cardiff, UK.

British Journal of Biomedical Science
|September 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sub-Chronic Peroneal Nerve Stimulation Lowers Ambulatory Blood Pressure in Spontaneously Hypertensive Rats.

IEEE open journal of engineering in medicine and biology·2024
Same author

Recombinant inbred lines derived from wide crosses in Pisum.

Scientific reports·2023
Same author

A dynamically coherent pattern of rhythms that matches between distant species across the evolutionary scale.

Scientific reports·2023
Same author

Waterlow score for risk assessment in surgical patients: a systematic review.

Annals of the Royal College of Surgeons of England·2021
Same author

Chondromalacia of the cranial medial femoral condyle; its occurrence and association with clinical outcome in a population of adult horses with stifle lameness.

Equine veterinary journal·2019
Same author

BEVA primary care clinical guidelines: Analgesia.

Equine veterinary journal·2019
Same journal

Artificial intelligence approaches in biological age prediction: current status and challenges.

British journal of biomedical science·2026
Same journal

Challenges and emerging strategies for genome-wide evaluation of loss of imprinting in cancer.

British journal of biomedical science·2026
Same journal

Application of non-invasive preimplantation genetic screening for aneuploidy through spent embryo culture media analysis at 48 and 54 hours after embryo cleavage.

British journal of biomedical science·2026
Same journal

Training on PD-L1 scoring in non-small cell lung cancer with high intra- and inter-reader agreement: results of a worldwide microscopic/digital image-based training of 751 pathologists.

British journal of biomedical science·2026
Same journal

Performance of a quality control center supporting national antimicrobial resistance surveillance.

British journal of biomedical science·2026
Same journal

The Molecular Pathology of Non-Malignant Haematological Disease.

British journal of biomedical science·2026
See all related articles

Dimethyl sulfoxide (DMSO) in paraffin wax enhances processing fluid penetration in histological samples. This method optimizes tissue processing times compared to pure paraffin wax, improving efficiency in histology.

Area of Science:

  • Histology
  • Biomedical Engineering
  • Materials Science

Background:

  • Histological processing requires efficient fluid penetration for accurate tissue analysis.
  • Standard paraffin wax embedding can limit the speed and depth of processing fluid infiltration.
  • Optimizing infiltration is crucial for reducing processing times and improving diagnostic yield.

Purpose of the Study:

  • To develop and evaluate a novel method for monitoring processing fluid penetration in histological tissues.
  • To assess the impact of incorporating dimethyl sulfoxide (DMSO) into paraffin wax on tissue processing.
  • To compare the efficacy of DMSO-modified paraffin wax with conventional paraffin wax.

Main Methods:

  • A new method was established to monitor the penetration of processing fluids into tissues.

Related Experiment Videos

  • Dimethyl sulfoxide (DMSO) was incorporated into paraffin wax formulations.
  • Processing times using DMSO-modified paraffin wax were compared against those using pure paraffin wax.

    • Main Results:

    • The incorporation of DMSO into paraffin wax was shown to facilitate processing fluid penetration.
    • Tissue processing times were significantly reduced when using DMSO-modified paraffin wax.
    • The method allows for effective monitoring during histological processing.

    Conclusions:

    • Dimethyl sulfoxide (DMSO) is an effective additive for improving paraffin wax-based histological processing.
    • This method enhances fluid penetration and reduces processing times, offering a more efficient approach to tissue preparation.
    • The developed technique aids in monitoring infiltration, crucial for consistent histological outcomes.