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Vitamin D3 elicits calcium response and activates blood monocyte-derived macrophages from patients with vitamin D
E Takeda1, K Takata, H Yamanaka
1Department of Clinical Nutrition, School of Medicine, The University of Tokushima, Tokushima City, Japan.
Abstract:
We studied the effects of vitamin D3 metabolites on intracellular free Ca2+ concentration ([Ca2+]i) and the respiratory burst of monocyte-derived macrophages (MDM) from patients with vitamin D dependent rickets type II. Treatment of MDM from the patients and healthy donors with 1 nM 1,25(OH)2D3 produced a rapid elevation of [Ca2+]i and similarly primed both types of cells for enhanced capacity for O2- release with phorbol diester. These results suggest that macrophages may have distinct non-genomic pathways of vitamin D3, which partly explain the absence of immunodeficiency and the disappearance of rickets after treatment with vitamin D3 in the patients.
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