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Protein components of a rat brain synaptic junctional protein preparation
K Langnaese1, C Seidenbecher, H Wex
1Federal Institute for Neurobiology, Department of Neurochemistry and Molecular Biology, Magdeburg, Germany.
Brain Research. Molecular Brain Research
|November 1, 1996
Abstract:
Antisera against a rat brain synaptic protein preparation, the postsynaptic density (PSD) fraction, were used to isolate cDNA clones by expression screening of a rat brain cDNA library. About one fifth of more than 200 analyzed cDNAs encoding potential synapse-associated proteins were previously unknown. Identifiable proteins include, among others, components of the pre- and postsynaptic cytoskeleton, synaptic vesicle proteins and several protein kinases and kinase substrates. This demonstrates that both pre- and postsynaptic elements purify with the PSD fraction.