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Enteroviral polymerase chain reaction in the investigation of aseptic meningitis

M H Riding1, J Stewart, G B Clements

  • 1Scottish Centre for Infection and Environmental Health, Ruchill Hospital, Glasgow, Scotland.

Journal of Medical Virology
|October 1, 1996
PubMed

Insights

Nested polymerase chain reaction (nPCR) significantly improves aseptic meningitis diagnosis by detecting more enteroviruses than traditional methods. This molecular technique enhances laboratory identification of enteroviral infections.

Area of Science:

  • Virology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Aseptic meningitis can be caused by enteroviruses.
  • Current diagnostic methods may fail to detect all enteroviral infections.

Purpose of the Study:

  • To evaluate the utility of nested polymerase chain reaction (nPCR) for diagnosing enteroviral meningitis.
  • To compare nPCR with conventional virus isolation techniques.

Main Methods:

  • Nested polymerase chain reaction (nPCR) was employed to detect enteroviruses.
  • Clinical samples from patients with aseptic meningitis symptoms were analyzed.
  • Results were compared to conventional virus isolation methods.

Main Results:

  • nPCR increased the detection rate of enteroviruses from 6% to 27% compared to isolation methods.
  • A total of 366 clinical samples collected between 1994-1995 were analyzed.
  • nPCR identified enteroviruses missed by conventional isolation.

Conclusions:

  • nPCR is a valuable tool for the laboratory diagnosis of enteroviral meningitis.
  • This molecular method significantly enhances the detection of enteroviruses.
  • nPCR can identify enteroviruses undetectable by current isolation techniques.

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