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The bacteriophage P1 lytic replicon: directionality of replication and cis-acting elements

G Cohen1, E Or, W Minas

  • 1Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, University of Tel-Aviv, Ramat Aviv, Israel.

Gene
|October 10, 1996
PubMed
Summary

Bacteriophage P1 lytic replication proceeds bidirectionally. The P53 promoter or repL protein likely functions in cis, meaning it acts locally, to enable replication.

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Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Bacteriophage P1 is a model organism for studying viral replication.
  • Understanding the mechanisms of viral DNA replication is crucial for controlling viral infections.

Purpose of the Study:

  • To determine the direction of bacteriophage P1 lytic replication.
  • To investigate the functional role of the P53 promoter and repL gene in P1 lytic replication.

Main Methods:

  • Construction of lambda P1 lysogens containing a functional P1 lytic replicon.
  • Analysis of bacterial DNA marker amplification to infer replication direction.
  • Complementation assays using wild-type and mutant P1 replicons with transposon insertions.

Main Results:

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  • Bacterial DNA markers flanking the lambda prophage were coordinately amplified, indicating bidirectional lytic replication.
  • A wild-type P1 replicon could not complement a mutant replicon with a transposon insertion between the P53 promoter and the repL gene.

Conclusions:

  • Bacteriophage P1 lytic replication occurs in a bidirectional manner.
  • The P53 promoter and/or the repL protein likely function in cis to facilitate P1 lytic replication.