Analysis of pseudorabies virus genes by cDNA sequencing
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Published on: July 27, 2018
T Y Ho1, C Y Hsiang, T J Chang
1Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan, ROC.
Abstract:
Cloning and sequencing of cDNA could provide a complementary approach to functional analysis of the pseudorabies virus (PrV) genome. Using colony hybridization, Southern hybridization, and DNA sequencing, four species of PrV-specific cDNA were identified. Among these four species of PrV-specific cDNA, three unidentified genes, UL26, UL29, and UL31, were mapped and a novel gI-11K bicistronic cDNA was confirmed. Thus, analysis of PrV-specific transcripts provided a way for identifying genes and a foundation to further study the roles of these transcripts in PrV infection.
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