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Characterization of the RNA dependent DNA polymerase of bovine leukemia virus
I Demirhan1, V Altanerova, A Chandra
1Laboratory of Molecular Biology (ZBC), University Medical School, Frankfurt am Main, Germany.
Anticancer Research
|September 1, 1996
Abstract:
The reverse transcriptase-RNA dependent DNA polymerase of Bovine Leukemia Virus (BLV) was isolated and characterized. The enzyme has a molecular weight of about 80kd and the isoelectric point is 7.6. The enzyme prefers magnesium, as a divalent cation using synthetic homopolymeric template primer poly (C) oligo (dG). Monoclonal antibodies directed against reverse transcriptase of human immunodeficiency virus type I (HIV-I) did not crossreact with the isolated polymerase.