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Neoplastic human tissue mast cells express the adhesion molecule CD44/HCAM
H P Horny1, D M Menke, E Kaiserling
1Institut für Pathologie, Universität Tübingen, Germany.
Virchows Archiv : an International Journal of Pathology
|October 1, 1996
Summary
This study investigated CD44 expression in human tissue mast cells (TMC). All normal, reactive, and neoplastic TMC showed strong CD44 immunoreactivity, suggesting diagnostic value for identifying atypical TMC in mastocytosis.
Area of Science:
- Immunohistochemistry
- Cell Biology
- Oncology
Background:
- Tissue mast cells (TMC) play roles in normal physiology and disease.
- CD44 (homing-associated cell adhesion molecule/HCAM) is a cell surface receptor involved in cell adhesion and migration.
- Understanding CD44 expression in TMC is crucial for diagnosing mast cell disorders.
Purpose of the Study:
- To determine the expression pattern of CD44 in normal, reactive, and neoplastic human tissue mast cells.
- To evaluate the diagnostic utility of CD44 immunohistochemistry in mast cell disorders.
Main Methods:
- Immunohistochemical analysis of CD44 expression using the DAKO-DF1485 antibody.
- Study included 30 human tissue specimens with normal/reactive and neoplastic TMC.
- Specimens were sourced from bone marrow, skin, spleen, liver, lymph nodes, and jejunal mucosa.
Main Results:
- All examined TMC, encompassing normal, reactive, and neoplastic types, displayed strong annular immunostaining for CD44.
- Consistent CD44 immunoreactivity was observed across all specimen types.
- The antibody DAKO-DF1485 effectively detected CD44 in all TMC examined.
Conclusions:
- CD44 is consistently expressed in human TMC, irrespective of their reactive or neoplastic state.
- Immunohistochemical detection of CD44 may serve as a valuable diagnostic marker for atypical TMC.
- This finding supports the role of CD44 as a principal surface receptor for hyaluronan in TMC and their neoplastic counterparts.