Axenic in vitro culture of Schistosoma mansoni miracidia

Miracidia of Schistosoma mansoni have been grown axenically. The use of saline solution of 0.75% facilitated the shedding of epidermal plates which if occurred promote a rapid sporocystogenesis. Also, the use of a ratio of 1:3 (fetal calf serum and medium 199) in addition to chernin balanced salt solution (SBSS) was ideal for maintaining sporocysts up to 14 days. All the miracidia sheded their epidermal plates 2 hours post cultivation. At the end of the second week post cultivation, the daughter sporocysts measured 690u in length by 50u in width. The usage of plastic flat sided flasks with plenty of culture medium was very important for induction a proper growth of sporocysts because it was possible that adherence of sporocysts to each other might delay their growth.