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Immunization with plasmid DNA encoding for the measles virus hemagglutinin and nucleoprotein leads to humoral and
A I Cardoso1, M Blixenkrone-Moller, J Fayolle
1INSERM Unit 404 Immunity and Vaccination, Institut Pasteur de Lyon, France.
Abstract:
We have evaluated the DNA vaccination strategy for measles virus (MV) hemagglutinin (HA) and nucleoprotein (NP) genes. Plasmids encoding either the MV, HA, or NP proteins inoculated intramuscularly into Balb/c mice induced both humoral and CTL class I restricted responses. Antibody responses were not increased by multiple inoculations. The major antibody isotype induced by both the HA and NP was IgG2a consistent with a Th1 response. In contrast, immunization with a plasmid which directed the synthesis of a partially secreted form of HA gave mainly IgG1 antibodies. When the amount of DNA was reduced for the HA plasmid (1 or 10 microg/animal), although the antibody was not induced, a CTL response was observed.