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Related Experiment Videos

Slime producing Staphylococci from clinical specimens - a simple diagnostic test

S K Makhija1, S V Jalgaonkar, M M Kher

  • 1Bacteriology Section, Department of Microbiology, Government Medical College, Nagpur.

Indian Journal of Pathology & Microbiology
|April 1, 1995
PubMed
Summary

Coagulase-negative Staphylococci are emerging pathogens. Over 42% of isolates produced slime, aiding adherence and potentially indicating pathogenicity, suggesting routine testing in labs.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Pathogen Research

Background:

  • Coagulase-negative Staphylococci (CoNS) are increasingly recognized as significant pathogens.
  • Certain CoNS strains produce a slime layer, a viscous extracellular material.
  • This slime facilitates bacterial adherence to surfaces, contributing to infections.

Purpose of the Study:

  • To investigate the prevalence of slime production in clinical isolates of Coagulase-negative Staphylococci.
  • To assess the potential role of slime production as an indicator of pathogenicity for CoNS.

Main Methods:

  • Analysis of 101 isolates of Coagulase-negative Staphylococci obtained from various clinical specimens.
  • Identification and quantification of slime-producing strains within the isolate collection.

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Main Results:

  • Out of 101 CoNS isolates, 43 (42.5%) were identified as slime producers.
  • The prevalence of slime production varied across different clinical sample types, from 20% in peritoneal fluid to 66.6% in cerebrospinal fluid.

Conclusions:

  • Slime production is a common characteristic among clinical isolates of Coagulase-negative Staphylococci.
  • Testing for slime production may be a valuable tool for assessing the pathogenicity of CoNS strains.
  • Routine slime production testing in diagnostic laboratories is recommended for CoNS isolates.