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FGF-2 influences cell movements and gene expression during limb development

S Li1, R Anderson, A D Reginelli

  • 1Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana 70118, USA.

The Journal of Experimental Zoology
|March 1, 1996
PubMed
Summary

Fibroblast growth factor-2 (FGF-2) influences limb development by altering cell movement and interactions. Ectopic FGF-2 in the posterior limb bud disrupts normal outgrowth, leading to reduced skeletal elements and digit loss.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Regenerative Medicine

Background:

  • Fibroblast growth factor-2 (FGF-2) is implicated as a key ectodermal signal regulating limb development.
  • Understanding FGF-2's role is crucial for deciphering limb outgrowth and patterning mechanisms.

Purpose of the Study:

  • To investigate the effects of ectopic FGF-2 application on limb bud morphology and gene expression.
  • To determine how FGF-2 influences cell movement and interactions during limb morphogenesis.

Main Methods:

  • Ectopic application of FGF-2 to chick limb buds.
  • Analysis of apical ectodermal ridge (AER)-dependent gene expression (Sonic hedgehog [Shh]).
  • Cell tracing using the lipophilic dye DiI to track cell migration patterns.

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Main Results:

  • Ectopic FGF-2 in the posterior limb bud maintained AER-dependent Shh and zone of polarizing activity (ZPA) signaling.
  • Posterior FGF-2 application resulted in reduced skeletal element length, digit loss, and altered Shh and HoxD13 expression domains.
  • FGF-2 stimulated posterior limb bud cells to move proximally and distally, causing cell population bifurcation.

Conclusions:

  • FGF-2 plays a significant role in limb outgrowth by modulating cell movements and position-specific interactions.
  • These FGF-2-induced cellular changes are critical for normal limb morphogenesis.
  • The findings provide insights into the molecular mechanisms governing vertebrate limb development.