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Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA
M Briglia1, R I Eggen, W M de Vos
1Institute for Plant Protection, Wageningen, Netherlands.
Applied and Environmental Microbiology
|April 1, 1996
Abstract:
A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 10(2) cells per g.