Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Invariant chain and DM edit self-peptide presentation by major histocompatibility complex (MHC) class II molecules

J F Katz1, C Stebbins, E Appella

  • 1Department of Pathology, University of Chicago, Illinois 60637, USA.

The Journal of Experimental Medicine
|November 1, 1996
PubMed
Summary

Invariant chain (Ii) and DM influence major histocompatibility complex (MHC) class II function. DM negatively regulates peptide presentation by editing the repertoire of peptides displayed on MHC class II molecules.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A funnel approach to enable analyses of epitope-specific human CD4 T cells specific for influenza and SARS-CoV-2.

mBio·2026
Same author

Role of non-genomic androgen signalling in suppressing proliferation of fibroblasts and fibrosarcoma cells.

Cell death & disease·2014
Same author

The pluripotency factor nanog promotes breast cancer tumorigenesis and metastasis.

Oncogene·2013
Same author

Light transmission from a randomly rough dielectric diffuser: theoretical and experimental results.

Optics letters·2009
Same author

Comparison of surface scattering between identical, randomly rough metal and dielectric diffusers.

Optics letters·2009
Same author

PML enhances the regulation of p53 by CK1 in response to DNA damage.

Oncogene·2008

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Invariant chain (Ii) is crucial for MHC class II function, including peptide binding blocking and cellular localization.
  • DM is believed to facilitate the removal of the Ii-derived CLIP peptide from MHC class II molecules in endosomes, enabling subsequent peptide loading.

Purpose of the Study:

  • To investigate the functional roles of invariant chain (Ii) and DM in the context of major histocompatibility complex (MHC) class II function.
  • To evaluate the impact of Ii and DM on the surface expression of epitopes recognized by alloreactive T cells.

Main Methods:

  • Studied the consequences of invariant chain (Ii) and DM expression on MHC class II function.
  • Examined the impact of Ii and DM on surface expression of epitopes recognized by a panel of alloreactive T cells.

Related Experiment Videos

Main Results:

  • Most studied epitopes are influenced by both Ii and DM.
  • DM expression in antigen-presenting cells (APCs) can extinguish, rather than enhance, the surface expression of a significant fraction of peptide-MHC class II complexes.
  • DM antagonizes epitopes not specific for CLIP and can extinguish recognition of internally synthesized peptides.

Conclusions:

  • DM may possess a generalized peptide-editing function for MHC class II, beyond just CLIP removal.
  • DM's editing function can be positive or negative, indicating a specifying role in peptide display for CD4 T cell recognition.