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Aquaporin 1 expression during the cell cycle in A5 cells
C Delporte1, Z J Chen, B J Baum
1Clinical Investigations and Patient Care Branch, National Institutes of Health, Bethesda, Maryland 20892, USA.
Biochemical and Biophysical Research Communications
|November 12, 1996
Summary
Aquaporin 1 (AQP1) water channel expression fluctuates during the cell cycle in epithelial cells. AQP1 levels are highest in G0/G1 and decrease in S and G2/M phases.
Area of Science:
- Molecular biology
- Cell biology
- Biochemistry
Background:
- Aquaporin 1 (AQP1) is an integral membrane protein functioning as a water channel.
- AQP1 is encoded by a delayed early response gene.
Purpose of the Study:
- To investigate if aquaporin 1 expression varies during the cell cycle.
- To analyze AQP1 mRNA and protein levels in epithelial cells across different cell cycle phases.
Main Methods:
- Synchronization of A5 epithelial cells using a double thymidine block.
- Flow cytometric analysis to define cell cycle stages.
- Quantification of AQP1 mRNA and protein levels.
Main Results:
- Constitutive aquaporin 1 expression was observed in A5 cells.
- AQP1 mRNA and protein levels peaked during the G0/G1 phases.
- A significant decrease (40-60%) in AQP1 levels was noted as cells transitioned to S and G2/M phases.
Conclusions:
- Aquaporin 1 expression is not static but fluctuates throughout the cell cycle.
- This study provides the first evidence of cell cycle-dependent regulation of constitutive aquaporin 1 expression.