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Evaluation of affinity constants by competitive binding assays: removal of empiricism from the Müller method
1Department of Biochemistry, University of Queensland, Brisbane, Australia.
Abstract:
A method is described for the quantitative screening of antigen analogs by competitive radioimmunoassay. This analytical procedure is based on an experimental design developed for the comparison of binding affinities by means of an empirical quantitative expression (Müller, R. (1980) J. Immunol. Methods 92, 589-601). The present analysis, which allows retention of the same general approach without sacrifice of thermodynamic rigor, is illustrated by application to literature results obtained by radioimmunoassay screening of the interactions between antigen analogs and antibodies elicited in response to (i) a scorpion toxin and (ii) 8-hydroxy-2-deoxyguanosine.