Localization of thrombomodulin in a rabbit eye
Related Experiment Video
Updated: Nov 3, 2026
Obtaining High-Quality Cryosections of Whole Rabbit Eye
Published on: November 10, 2023
H Kawamura1, Y Hiramatsu, I Watanabe
1Department of Ophthalmology, Hamamatsu University School of Medicine, Shizuoka, Japan.
Purpose:
To show the distribution of an anticoagulant factor, thrombomodulin, in a rabbit eye.
Methods:
Immunohistochemical staining using polyclonal anti-rabbit thrombomodulin antibody was performed with the excised eyes of pigmented adult rabbits. Streptavidin-biotin method was used to obtain intense staining.
Results:
The endothelium of the chorioretinal vessels showed positive linear staining. The ciliary non-pigmented epithelium showed granular staining, while the pigmented epithelium stained negatively. The positive staining was also detected in the trabecular plexus endothelium. The vascular endothelium of the ciliary body stained weakly.
Conclusions:
It is suggested that thrombomodulin in the vessel walls and the aqueous drainage apparatus works to maintain normal blood fluidity and aqueous outflow. The role of nonvascular thrombomodulin in the ciliary non-pigmented epithelium is unclear.
