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Immunocytochemical localization of retinoid-binding protein in the dogfish retina
1Department of Ophthalmology, Northwestern University Medical School, Chicago, IL 60611, USA.
Purpose:
The cellular localization of retinoid-binding proteins has been extensively studied in the mammalian retina. Less is known about the distribution and identity of retinoid-binding proteins in lower vertebrate retinas. To understand their cellular functions, we have examined the distribution of specific retinoid-binding proteins in the neural retina and RPE of an elasmobranch, the dogfish.
Methods:
Retinoid-binding proteins were localized in cryostat sections of dogfish eyes by the indirect immunofluorescence technique. We used well-characterized antibodies that have been previously employed for immunostaining. Immunoblotting was carried out to identify the antigen.
Results:
The studies show that the cellular retinaldehyde-binding protein is present in the RPE and retinal Müller cells, and the interphotoreceptor retinoid-binding protein is found in the interstitial space between pigment epithelium and retina.
Conclusions:
The distribution of retinoid-binding proteins in dogfish retina is similar to that found in the mammalian retina which suggests that the sites of action of the retinoid-binding proteins are highly conserved in vertebrates.