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Topical anti-inflammatory lignans from Haplophyllum hispanicum
J M Prieto1, M C Recio, R M Giner
1Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Spain.
Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|September 1, 1996
Summary
This study isolated two anti-inflammatory compounds, diphyllin acetyl apioside and tuberculatin, from Haplophyllum hispanicum. Diphyllin acetyl apioside showed significant activity against topical inflammation in mice.
Area of Science:
- Pharmacology
- Natural Products Chemistry
Background:
- Inflammation is a complex biological response.
- Natural products are a source of potential anti-inflammatory agents.
Purpose of the Study:
- To investigate the anti-inflammatory potential of Haplophyllum hispanicum methanol extract.
- To isolate and identify active compounds responsible for the anti-inflammatory effects.
Main Methods:
- Methanol extraction of Haplophyllum hispanicum.
- Topical administration of 12-O-tetradecanoylphorbol acetate (TPA) and oxazolone to induce inflammation in mouse ear edema models.
- Isolation of compounds using spectroscopic methods (13C NMR, HMBC).
Main Results:
- Two aryl naphthalide lignans, diphyllin acetyl apioside and tuberculatin, were isolated.
- Diphyllin acetyl apioside demonstrated potent anti-inflammatory activity in acute TPA-induced edema, with an ID50 of 0.27 μmol/ear.
Conclusions:
- Haplophyllum hispanicum methanol extract possesses significant anti-inflammatory properties.
- Diphyllin acetyl apioside is a promising topical anti-inflammatory agent derived from natural sources.