Related Experiment Videos
A set of compatible tac promoter expression vectors
D M Dykxhoorn1, R St Pierre, T Linn
1Department of Microbiology and Immunology, Faculty of Medicine, University of Western Ontario, London, Canada.
Gene
|October 24, 1996
Abstract:
A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacIq gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.