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Escherichia coli strain for thermoinducible T7 RNA polymerase-driven expression
N D Fedorova1, M Y Peredelchuk, M P Kirpichnikov
1Engelhardt Institute of Molecular Biology, Moscow, Russia.
Gene
|October 24, 1996
Abstract:
An Escherichia coli strain for thermoinducible T7Pol-driven transcription has been constructed. The strain was obtained by site-specific integration of the T7 gene 1 coding for T7Pol into the attB site of phage lambda in the E. coli chromosome. The expression of the inserted gene is regulated by cIts857 and major early promoter-operator regions of phage lambda.