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The use of sequential affinity chromatography for separation of human neutrophil elastase, cathepsin G and azurocidin
W Watorek1, A Polanowski, T Wilusz
1Institute of Biochemistry, Wrocław University, Poland.
Acta Biochimica Polonica
|January 1, 1996
Abstract:
Elastase, cathepsin G and azurocidin from human neutrophils are key components of body inflammatory defense. Perturbations in regulation of their activities lead to many serious pathological states. The paper describes a simple, fast and efficient method of joint purification of these proteins with the use of sequential affinity chromatography on squash trypsin inhibitor (CMTI I) and bovine pancreatic trypsin inhibitor (BPTI).