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Characterization of Vibrio cholerae EIT or typing phage D10
B K Chakrabarti1, K Si, D Chattopadhyay
1Department of Biochemistry, University College of Science, Calcutta, India.
The Journal of General Virology
|November 1, 1996
Summary
This study characterizes the Vibrio cholerae typing phage D10, detailing its adsorption, intracellular growth, and DNA. Phage yield is influenced by inhibitors, and its DNA structure was mapped.
Area of Science:
- Microbiology
- Virology
- Molecular Biology
Background:
- Phage typing is crucial for identifying and differentiating bacterial strains.
- Vibrio cholerae causes cholera, a significant global health concern.
Purpose of the Study:
- To characterize the Vibrio cholerae EITor typing phage D10.
- To elucidate its adsorption kinetics, intracellular growth, and DNA properties.
Main Methods:
- Adsorption kinetics assays.
- Intracellular growth studies (eclipse, latent period, burst size).
- DNA analysis including size, structure, and restriction mapping (BamHI, HindIII, PstI).
Main Results:
- Biphasic adsorption kinetics observed.
- Eclipse period (20 min), latent period (25 min), and burst size (80 particles/cell) determined.
- Phage yield affected by DNA/RNA synthesis inhibitors (nalidixic acid, novobiocin, rifampicin).
- Characterized as a 32+/-0.2 kb linear double-stranded DNA molecule with unique termini.
Conclusions:
- Vibrio cholerae typing phage D10 exhibits specific growth and adsorption characteristics.
- Phage replication is sensitive to host DNA and RNA synthesis inhibition.
- Detailed molecular characterization provides a foundation for its use in phage typing and further research.