Related Experiment Videos
Expression and polarization of VGF in developing hippocampal neurons
1Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Brain Research. Developmental Brain Research
|October 23, 1996
Summary
VGF protein expression, found in vesicles, correlates with neurite outgrowth in PC12 cells and hippocampal neurons. VGF is particularly abundant in GABAergic neurons and becomes restricted to axons during development.
Area of Science:
- Neuroscience
- Cell Biology
Background:
- VGF is a nerve growth factor-inducible protein.
- VGF expression correlates with neurite outgrowth in PC12 cells.
- VGF is localized to large dense core vesicles.
Purpose of the Study:
- To investigate the role of VGF in neural and axonal differentiation.
- To examine VGF immunoreactivity in developing hippocampal neurons.
Main Methods:
- Immunolocalization using electron microscopy in PC12 cells.
- Examination of VGF immunoreactivity in cultured hippocampal neurons.
- Colocalization studies with synaptophysin.
Main Results:
- VGF is present in vesicles in developing hippocampal neurons from early stages.
- VGF is particularly abundant in a subset of GABAergic neurons.
- VGF expression becomes restricted to axons after dendrite maturation.
- VGF-containing vesicles are distinct from synaptophysin-containing vesicles.
Conclusions:
- VGF plays a role in neuronal differentiation and axonal development.
- VGF localization suggests involvement in specific neuronal populations and processes.
- VGF and synaptophysin mark distinct vesicle populations.