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Related Experiment Videos

[Oocyte maturation and intracytoplasmic sperm injection]

J Mandelbaum1, A M Junca, J Belaisch-Allart

  • 1Hôpital Necker, Paris.

Contraception, Fertilite, Sexualite (1992)
|July 1, 1996
PubMed
Summary

Intracytoplasmic sperm injection (ICSI) aids in assessing oocyte maturity and fertilizing immature eggs. While in vitro maturation and ICSI show promise, further research is needed to confirm embryonic viability and pregnancy rates.

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Area of Science:

  • Reproductive endocrinology and infertility
  • Assisted reproductive technologies
  • Oocyte biology

Background:

  • Intracytoplasmic sperm injection (ICSI) is a key treatment for severe male infertility.
  • Oocyte maturity assessment is crucial for successful assisted reproduction.
  • Immature oocytes retrieved during ovarian stimulation pose a challenge in IVF.

Purpose of the Study:

  • To evaluate the feasibility of in vitro maturation and subsequent fertilization of immature oocytes retrieved during ICSI cycles.
  • To assess the fertilization potential of immature oocytes matured in vitro compared to in vivo mature oocytes.
  • To explore the role of ICSI in overcoming oocyte maturation anomalies.

Main Methods:

  • Oocytes were retrieved 34 hours post-hCG from follicles > 14 mm in cycles using GnRH analog desensitization and hMG/FSH stimulation.

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  • Immature oocytes (prophase I or metaphase I) were cultured in vitro to assess their ability to achieve meiosis.
  • Fertilization was confirmed by the presence of two pronuclei (2 PN) after ICSI.

    • Main Results:

    • 12% of aspirated oocytes were immature at recovery.
    • 66% of immature oocytes achieved meiosis in vitro.
    • 51% of in vitro matured oocytes were successfully fertilized by ICSI, comparable to in vivo mature oocytes.

    Conclusions:

    • ICSI enables accurate oocyte maturity evaluation and fertilization of immature oocytes.
    • In vitro maturation followed by ICSI offers a viable option for immature oocytes, though embryonic viability requires further assessment.
    • ICSI is essential for fertilization in cases of specific oocyte maturation defects, preventing polyspermy.