[Gene K-ras mutation in cells of endometrial adenocarcinoma in women]

A simple, sensitive method of DNA analysis of nucleotide substitutions, namely, single-strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP analysis) was used for detection of mutated K-ras gene in specimens of human endometrial cancer. The mobility shifts of single-strand DNA fragment was seen in 3 of 8 analysed neoplasms: two in stage I and one in stage II, according to FIGO classification, K-ras gene mutation was found in 3 out of 4 patients over 66 years old.