Lipogenesis in hepatocytes of genetically obese rats

A simple method for the preparation of hepatocytes in good yield from obese rats is described. Lipogenesis from [U-14C]-lactate, [U-14C]-glucose and tritiated water has been investigated in hepatocytes prepared from both genetically obese 'fatty' rats and their lean littermates. Hepatocytes from obese rats demonstrate elevated rates of fatty acid and sterol synthesis in contrast to hepatocytes from lean animals. The rate of fatty acid synthesis is sensitive to the dietary status of the animal prior to preparation. The enhanced lipogenesis in hepatocytes from obese rate depends on lactate as a source of carbon rather than glucose. The role of the liver in genetic obesity is discussed with particular reference to the major precursor for hepatic lipogenesis.