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Related Experiment Videos

Decrease in protein tyrosine phosphatase activities in vanadate-treated obese Zucker (fa/fa) rat liver

S Pugazhenthi1, F Tanha, B Dahl

  • 1Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.

Molecular and Cellular Biochemistry
|December 6, 1995
PubMed
Summary
This summary is machine-generated.

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Vanadate treatment reduces insulin levels and increases insulin receptors in obese rats. This study shows vanadate

Area of Science:

  • Biochemistry
  • Pharmacology
  • Endocrinology

Background:

  • Vanadate's insulin-like effects are linked to protein tyrosine phosphatase (PTPase) inhibition.
  • Obese Zucker rats exhibit hyperinsulinemia, hyperglycemia, and reduced insulin receptors.

Purpose of the Study:

  • To investigate the in vivo effects of vanadate on hepatic PTPases in obese Zucker rats.
  • To determine vanadate's impact on insulin receptor numbers and plasma insulin levels.

Main Methods:

  • Chronic vanadate administration to obese Zucker rats.
  • Measurement of hepatic PTPase activity using specific substrates.
  • Analysis of insulin receptor numbers and plasma insulin levels.
  • Chromatographic separation of PTPase activity using Mono Q column.

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Main Results:

  • Vanadate treatment decreased plasma insulin by 80% and increased insulin receptor numbers in obese rats.
  • Hepatic PTPase activity, elevated in obese rats, significantly decreased after vanadate administration.
  • Chromatographic data confirmed the reduction in PTPase activity.

Conclusions:

  • Vanadate's insulin-mimetic action is associated with the inhibition of hepatic protein tyrosine phosphatases.
  • Targeting PTPases may represent a therapeutic strategy for managing type II diabetes and obesity.