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Periprandial systemic and regional lipase activity in normal humans
S W Coppack1, T J Yost, R M Fisher
1Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota 55905, USA.
The American Journal of Physiology
|April 1, 1996
Summary
This study reveals distinct regulation of lipoprotein lipase (LPL) release from adipose and muscle tissues. Post-meal, adipose tissue releases more LPL, while muscle tissue releases less, impacting triglyceride clearance.
Area of Science:
- Metabolism
- Physiology
- Biochemistry
Background:
- Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) are key enzymes in triglyceride metabolism.
- Understanding tissue-specific LPL regulation is crucial for metabolic health.
Purpose of the Study:
- To investigate arteriovenous differences in LPL and HTGL activity across skeletal muscle and adipose tissue.
- To determine how LPL release from these tissues is regulated in postabsorptive and postprandial states.
Main Methods:
- Assay for plasma LPL activity without prior heparin injection.
- Measurement of LPL and HTGL activities and triglyceride concentrations in arterial and venous plasma from forearm muscle and abdominal adipose tissue.
- Comparison of measurements in postabsorptive and postprandial states.
Main Results:
- Triglyceride clearance was higher in adipose tissue compared to skeletal muscle.
- No arteriovenous differences were observed for HTGL activity.
- Skeletal muscle released LPL in the postabsorptive state, while adipose tissue did not.
- Postprandially, adipose tissue LPL activity increased, whereas muscle LPL activity decreased.
Conclusions:
- Release of LPL from subcutaneous adipose and forearm muscle tissues is differentially regulated.
- These findings highlight in vivo differences in LPL regulation at the tissue level, impacting postprandial lipid metabolism.