Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane junctions associated with rubella virus infected cells

J Y Lee1, D S Bowden, J A Marshall

  • 1Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.

Journal of Submicroscopic Cytology and Pathology
|January 1, 1996
PubMed
Summary

Rubella virus (RV) infection causes unique membrane fusions near replication sites and mitochondria. These specialized membrane junctions suggest a link between viral replication energy demands and cellular structures.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genotype analysis of noroviruses associated with gastroenteritis outbreaks in childcare centres, Victoria, Australia, 2012-2015.

Epidemiology and infection·2017
Same author

The effects of needle-sharing and opioid substitution therapy on incidence of hepatitis C virus infection and reinfection in people who inject drugs.

Epidemiology and infection·2016
Same author

Vidal LL, et al. NS3 protease polymorphisms and genetic barrier to drug resistance of distinct HCV genotypes from worldwide treatment-naive subjects. JVH, 2016, 23(11): 840-849.

Journal of viral hepatitis·2016
Same author

The relationships between IFNL4 genotype, intrahepatic interferon-stimulated gene expression and interferon treatment response differs in HCV-1 compared with HCV-3.

Alimentary pharmacology & therapeutics·2015
Same author

Norovirus genotype diversity associated with gastroenteritis outbreaks in aged-care facilities.

Epidemiology and infection·2015
Same author

A novel hepatitis B virus S gene insertion associated with reduced humoral immunity and diagnostic escape.

Internal medicine journal·2014

Area of Science:

  • Cell Biology
  • Virology
  • Microscopy

Background:

  • Rubella virus (RV) is an important human pathogen.
  • Viral replication relies on host cell machinery, including membrane systems and energy supply.
  • Mitochondria are crucial for cellular energy production.

Purpose of the Study:

  • To investigate the ultrastructural changes in host cell membranes during rubella virus infection.
  • To characterize the novel membrane junctions formed in RV-infected cells.
  • To explore the relationship between viral replication complexes and mitochondria.

Main Methods:

  • Transmission electron microscopy (TEM) of infected Vero cells.
  • Comparative analysis of RV-infected, Semliki Forest virus (SFV)-infected, and mock-infected cells.

Related Experiment Videos

  • Morphological characterization of membrane structures.

    • Main Results:

    • RV infection induced striking membrane alterations around replication complexes and mitochondria.
    • Three distinct membrane fusion configurations were identified: CM-1, CM-2, and CC.
    • These specific junctions (CM-1, CM-2, CC) were unique to RV-infected cells.
    • Mitochondria clustered around replication complexes in both RV and SFV infections, indicating high energy demand.
    • Electron-dense zones formed continuous links between RV replication complexes and mitochondria.

    Conclusions:

    • Rubella virus infection triggers the formation of specialized membrane structures (CM-1, CM-2, CC).
    • These structures may facilitate the high energy requirements of viral replication by linking replication complexes to mitochondria.
    • The observed membrane dynamics highlight a novel interaction between RV and host cell organelles.