Related Experiment Video

Updated: Aug 12, 2026

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

Preparation of carboxyl-terminal tryptic peptides from proteins by cleavage at arginine

S L Fong, P A Hargrave

    International Journal of Peptide and Protein Research
    |January 1, 1977
    PubMed

    More Related Videos

    Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT10:30

    Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

    Published on: September 2, 2015

    A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis09:40

    A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

    Published on: April 28, 2022

    Related Concept Videos

    Protein Digestion01:02

    Protein Digestion

    Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
    Preparation of Carboxylic Acids: Overview01:31

    Preparation of Carboxylic Acids: Overview

    There are various methods for the preparation of carboxylic acids. For example, oxidation of primary alcohols or aldehydes using strong oxidizing agents results in a carboxylic acid. Aldehydes can also be oxidized in the presence of mild oxidizing agents.
    Preparation of Amides01:29

    Preparation of Amides

    Amides are synthesized by treating carboxylic acids with amines in the presence of dehydrating agents like dicyclohexylcarbodiimide (DCC).
    The DCC-promoted synthesis of amides begins with the protonation of DCC by carboxylic acid. The protonation makes it a better acceptor. Next, the addition of carboxylate to the protonated carbodiimide gives a reactive acylating agent.
    Subsequently, the amine acts as a nucleophile that attacks the acylating agent to form a tetrahedral intermediate. In the...
    Amides to Carboxylic Acids: Hydrolysis01:28

    Amides to Carboxylic Acids: Hydrolysis

    Amides can undergo either acid-catalyzed hydrolysis or base-promoted hydrolysis through a typical nucleophilic acyl substitution. Each hydrolysis requires severe conditions.
    Acid-catalyzed hydrolysis:
    Hydrolysis of amides under acidic conditions yields carboxylic acids. Since the reaction occurs slowly, hydrolysis requires the conditions of heat.
    The mechanism begins with the protonation of the carbonyl oxygen by the acid catalyst. The protonation makes the amide carbonyl carbon more...
    Preparation of Carboxylic Acids: Hydrolysis of Nitriles01:19

    Preparation of Carboxylic Acids: Hydrolysis of Nitriles

    Nitriles (R–CN) can be converted into carboxylic acids (R–COOH) upon treatment with aqueous acids, i.e., upon hydrolysis of nitriles. Under base-catalyzed conditions, carboxylate anions (R–COO−) are formed.
    Preparation of Carboxylic Acids: Carboxylation of Grignard Reagents01:13

    Preparation of Carboxylic Acids: Carboxylation of Grignard Reagents

    Carboxylic acids can be prepared by the carboxylation of Grignard reagents (RMgX). This method is convenient for converting alkyl (primary, secondary or tertiary), vinyl, benzyl, and aryl halides to carboxylic acids with one additional carbon than the starting RMgX.