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Evaluation of two microhematocrit methods for quantitating plasma fibrinogen

F S Blaisdell, W J Dodds

    Journal of the American Veterinary Medical Association
    |August 15, 1977
    PubMed
    Summary

    Two heat-precipitation assays for plasma fibrinogen in animals significantly underestimated levels compared to a reference method. The Millar et al assay showed better reproducibility and is recommended for clinical use.

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    Area of Science:

    • Veterinary Hematology
    • Clinical Pathology
    • Biochemistry

    Background:

    • Accurate quantitation of plasma fibrinogen is crucial for diagnosing and monitoring various animal diseases.
    • Existing methods for plasma fibrinogen measurement vary in complexity and reliability.
    • Heat-precipitation assays offer a potentially simpler alternative for fibrinogen determination.

    Purpose of the Study:

    • To compare the accuracy and reproducibility of two heat-precipitation assays (Millar et al. and Schalm et al.) against a reference method (Ratnoff-Menzie) for plasma fibrinogen quantitation in multiple animal species.
    • To evaluate the suitability of the Millar et al. assay for routine clinical use in veterinary diagnostics.

    Main Methods:

    • Blood samples were collected from various animal species, including humans.

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  • Plasma fibrinogen levels were measured using the Ratnoff-Menzie reference assay.
  • Plasma fibrinogen was also quantitated using the Millar et al. and Schalm et al. heat-precipitation assays.
  • Assay performance was evaluated based on accuracy (comparison to reference) and reproducibility.

    • Main Results:

    • Both heat-precipitation assays significantly underestimated plasma fibrinogen levels when compared to the Ratnoff-Menzie reference assay.
    • The Millar et al. assay demonstrated significantly better reproducibility than the Schalm et al. assay.
    • The reproducibility of the Millar et al. assay closely approached that of the Ratnoff-Menzie method.
    • The Millar et al. assay requires no specialized equipment, making it practical for clinical settings.

    Conclusions:

    • Heat-precipitation methods, particularly the Schalm et al. assay, tend to underestimate plasma fibrinogen concentrations in animals.
    • The Millar et al. heat-precipitation assay offers acceptable reproducibility and practicality for clinical veterinary use.
    • The Millar et al. assay is recommended for routine clinical assessment of plasma fibrinogen in animals due to its simplicity and reasonable accuracy.