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The amino acid sequence and structural class of the arachin subunit of molecular weight 29, 100A
R Bhushan1, V K Mahesh, A Varma
1Department of Chemistry, University of Roorkee, India.
Biochemistry and Molecular Biology International
|February 1, 1996
Abstract:
Arachin subunit (mol. wt. 29,100) from peanut of variety G-201 was separated and isolated, its purity and homogeneity were ascertained. The subunit was cleaved with trypsin, chymotrypsin and Staphylococcus aureus V8 protease. The fragments obtained were separated and isolated by PAGE, gel filtration & ion exchange chromatography, these were subjected to amino acid analysis, and Edman degradation. The PTH amino acids obtained were identified by spectroscopy and TLC. The complete amino acid sequence of the subunit (226 residues) was established, and the structural class of arachin was predicted from the amino acid composition.