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Related Experiment Videos

Quantitative nephelometric assay for determining alpha-foetoprotein evaluated

D R Bernard1, J R Delanghe, M L De Buyzere

  • 1Laboratorium voor Klinische Biologie, Afdeling Klinische Scheikunde, Universitair Ziekenhuis Gent, Belgium.

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|October 1, 1996
PubMed
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This study validates a new automated nephelometric immunoassay for alpha-foetoprotein (AFP) measurement. The method demonstrates excellent stability, precision, and accuracy, proving reliable for clinical use in serum and amniotic fluid.

Area of Science:

  • Clinical Chemistry
  • Immunochemistry
  • Analytical Biochemistry

Background:

  • Accurate measurement of alpha-foetoprotein (AFP) is crucial for prenatal diagnostics and tumor monitoring.
  • Existing immunoassays may face limitations in terms of speed, practicality, or interference.
  • Automated nephelometric immunoassays offer potential for improved efficiency and reliability.

Purpose of the Study:

  • To evaluate a novel automated nephelometric immunoassay for AFP determination in serum and amniotic fluid.
  • To assess the method's stability, precision, accuracy, and potential interferences.
  • To compare the performance against a commercial radioimmunoassay (RIA).

Main Methods:

  • Evaluation of an automated nephelometric immunoassay using shell/core particles on the Behring Nephelometer analyser II.

Related Experiment Videos

  • Assessment of method stability, intra-assay and inter-assay coefficients of variation (CV).
  • Interference studies with haemoglobin, bilirubin, rheumatoid factors, human anti-mouse antibodies, and triacylglycerols; correlation analysis with RIA; investigation of molecular variations and multimeric forms using chromatography.

    • Main Results:

    • The method exhibited good stability (reagents and calibration curve stable for ≥1 week) and precision (intra-assay CV 2.3–4.0%, inter-assay CV 3.5–4.6%).
    • No high-dose hook effect observed up to 273000 µg/l; minimal interference from common substances, except for chylomicrons at low triacylglycerol levels.
    • Excellent correlation with RIA for both serum (n=65) and amniotic fluid (n=100); molecular variations and autopolymerization had negligible effects.

    Conclusions:

    • Latex-enhanced immunonephelometry is a rapid, practical, and reliable method for measuring AFP in serum and amniotic fluid.
    • The automated assay demonstrates robustness against common interfering substances and molecular variations.
    • This method is suitable for routine clinical laboratory use, offering high accuracy and efficiency.