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Related Experiment Videos

pH-dependent microspheres from modified soybean protein hydrolysate

S J Milstein1, E N Barantsevitch, V A Grechanovski

  • 1Emisphere Technologies, Inc., Hawthorne, NY 10532, USA.

Journal of Microencapsulation
|November 1, 1996
PubMed
Summary
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Modified soybean protein forms pH-dependent microspheres for drug delivery. These novel microspheres successfully encapsulated and delivered proteins orally in rats, showing potential for enhanced therapeutic applications.

Area of Science:

  • Biomaterials Science
  • Protein Chemistry
  • Drug Delivery Systems

Background:

  • Soybean hydrolysate, a hydrophilic mixture, is soluble across all pH levels.
  • Chemical modification can alter protein properties for specific applications.

Purpose of the Study:

  • To chemically modify soybean hydrolysate to create pH-dependent microspheres.
  • To characterize the physiochemical properties of these novel microspheres.
  • To evaluate their potential for encapsulating and delivering proteins orally.

Main Methods:

  • Chemical modification of soybean hydrolysate with aromatic acyl chlorides.
  • Analysis of physiochemical properties including hydrophobic/hydrophilic ratio, molecular weight, and internal structure using HPLC, sedimentation, and light scattering.

Related Experiment Videos

  • Encapsulation of porcine insulin and salmon calcitonin.
  • Oral gavage studies in rats to assess systemic circulation of encapsulated proteins.

    • Main Results:

    • Acylation of soybean hydrolysate yielded pH-dependent microspheres with altered hydrophobic/hydrophilic ratios.
    • Microspheres exhibited an average molecular weight of 10(7)-10(8) Daltons and contained discrete internal chambers.
    • Nearly 60% of encapsulated proteins (porcine insulin, salmon calcitonin) were delivered, achieving significant titers in the systemic circulation of rats after oral administration.

    Conclusions:

    • Modified soybean protein can form pH-dependent, supramolecular microspheres.
    • These microspheres demonstrate potential as a delivery vehicle for oral protein therapeutics.
    • The hydrophobic/hydrophilic ratio and low molecular weight are critical factors for microsphere formation.