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Selective plasmid transduction in Bacillus pumilus

M G Bramucci, P S Lovett

    Journal of Bacteriology
    |September 1, 1977
    PubMed
    Summary
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    The bacteriophage phi75 efficiently transduces Bacillus pumilus plasmids but not chromosomal DNA. This suggests phi75 is a specialized transducing phage for plasmid DNA transfer.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Bacteriophages are viruses that infect bacteria and can mediate horizontal gene transfer.
    • Transduction is a mechanism of genetic exchange mediated by bacteriophages.
    • Plasmid transduction is crucial for the spread of antibiotic resistance and other genetic traits.

    Purpose of the Study:

    • To investigate the transduction capabilities of the inducible temperate bacteriophage phi75 and its variant phi75C1.
    • To determine if phi75 can mediate the transfer of Bacillus pumilus plasmid pPL10 and chromosomal markers.
    • To characterize the properties of phi75 particles responsible for plasmid transduction.

    Main Methods:

    • Bacteriophage phi75 and its variant phi75C1 were used to infect Bacillus pumilus strains harboring the pPL10 plasmid.

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  • Transduction frequencies for plasmid pPL10 and chromosomal markers were determined by plaque assays.
  • Sedimentation velocity and buoyant density analyses were performed on phi75 particles.

    • Main Results:

    • Bacteriophage phi75 and phi75C1 mediated the transduction of the Bacillus pumilus plasmid pPL10 at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit.
    • Transduction of chromosomal markers by phi75 and phi75C1 was not detected at significant levels.
    • The plasmid transducing activity of phi75 was associated with particles exhibiting sedimentation velocity and buoyant density similar to infectious phage particles.

    Conclusions:

    • The temperate bacteriophage phi75 acts as a specialized transducing phage, efficiently transferring the multicopy plasmid pPL10 in Bacillus pumilus.
    • Phi75 does not appear to transduce chromosomal markers, indicating a preference for plasmid DNA.
    • The findings contribute to understanding phage-mediated gene transfer mechanisms, particularly for plasmids.