Related Experiment Video

Updated: Aug 15, 2026

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
12:07

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

Horsetail (Equisetum telmateia) ferredoxins I and II. Amino acid sequences

T Hase, K Wada, H Matsubara

    Journal of Biochemistry
    |July 1, 1977
    PubMed

    More Related Videos

    Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve09:13

    Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

    Published on: June 14, 2017

    Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group07:49

    Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

    Published on: August 16, 2017

    Related Concept Videos

    Globular and Fibrous Proteins02:21

    Globular and Fibrous Proteins

    Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
    Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
    Conserved Binding Sites01:49

    Conserved Binding Sites

    Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
    Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
    Electron Transport Chain: Complex I and II01:46

    Electron Transport Chain: Complex I and II

    The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
    ROS generation is regulated and maintained at moderate levels necessary...
    Mitochondrial Precursor Proteins01:39

    Mitochondrial Precursor Proteins

    Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
    Most of the mitochondrial precursors...
    Electron Transport Chain: Complex III and IV01:43

    Electron Transport Chain: Complex III and IV

    During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
    Sulfur Assimilation01:20

    Sulfur Assimilation

    Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...