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Related Experiment Videos

Human lymphoblastoid interferon. Large scale production and partial purification

P J Bridgen, C B Anfinsen, L Corley

    The Journal of Biological Chemistry
    |October 10, 1977
    PubMed
    Summary

    This study details the large-scale production and purification of human lymphoblastoid interferon (IFN-alpha). Researchers achieved significant purification and characterized the interferon

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    Area of Science:

    • Biotechnology
    • Virology
    • Immunology

    Background:

    • Human lymphoblastoid interferon (IFN-alpha) is a crucial antiviral protein.
    • Efficient large-scale production and purification methods are essential for therapeutic applications.

    Purpose of the Study:

    • To describe the large-scale production of human lymphoblastoid interferon.
    • To detail the purification process and characterize the interferon's properties.

    Main Methods:

    • Namalva cells were induced with Newcastle disease virus for interferon production.
    • Purification involved affinity chromatography, ion exchange chromatography, isoelectric focusing, and SDS-PAGE.
    • Antiviral activity and molecular weight were assessed using specific assays.

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    Main Results:

    • Interferon was produced on an 800-liter scale, yielding 2.6 X 10(9) units.
    • Purification achieved approximately 35,000-fold enrichment.
    • SDS-PAGE resolved activity into two components (18,000 and 22,000 Da), with glycosidase treatment localizing activity to the lower molecular weight species.

    Conclusions:

    • A robust method for large-scale human lymphoblastoid interferon production and purification was established.
    • Characterization revealed distinct molecular weight forms of interferon activity.
    • Effective recovery of interferon activity from SDS-PAGE gels was demonstrated.