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Chromosomal integration dependent induction of junB by growth factors requires multiple flanking evolutionarily
D G Phinney1, S W Tseng, B Hall
1Fox Chase Cancer Center, Philadelphia, Pennsylvania 19007, USA.
Oncogene
|November 7, 1996
Summary
Evolutionarily conserved sequences near the junB gene are crucial for its transcriptional regulation. Specific elements within these regions, including novel motifs, are essential for junB induction and chromatin structure.
Area of Science:
- Molecular Biology
- Genetics
- Gene Regulation
Background:
- The junB locus contains nine evolutionarily conserved sequences (FECS) conserved between human and mouse.
- These FECS are necessary for maximal junB induction and contain most known junB regulatory cis-elements.
Purpose of the Study:
- To identify specific regulatory cis-elements within junB FECS II and III.
- To quantitatively analyze the contribution of these sequences to junB induction.
Main Methods:
- Identification and mutation of specific cis-elements within junB FECS II and III.
- Quantitative analysis of junB induction following cis-element mutation.
- Analysis of chromatin structure alterations upon mutation.
Main Results:
- Specific regulatory cis-elements, including a Serum Response Element (SRE), two Ets sites, and two novel Ets-linked motifs (ELMs), were identified within junB FECS II and III.
- Mutation of any single identified cis-element significantly impaired junB induction.
- Mutations also altered the 5' flanking DNA chromatin structure of junB.
Conclusions:
- Multiple proteins binding within FECS form a functional promoter complex for junB.
- The activity of this promoter complex is dependent on a specific chromatin architecture.
- The evolutionary conservation of FECS highlights their critical role in junB transcriptional regulation.